The goal of this content is to serve as a resource for anyone wanting to learn more about long-term, live-cell imaging. Explore the resources below to learn more about how live-cell analysis outperforms other methods. Generate data rich information from every sample with fluorescent reagent combinations – compatible with a variety of cell culture models and applications Spend more time investigating and less time troubleshooting with lab-tested protocols and purpose-built, intuitive software Unlock 3D biology with ease using intuitive software and automated processes to acquire and analyze complex biology
However, only the Incucyte® SX5 and S3 support independent vessel scheduling for different image acquisition frequencies. All Incucyte® models provide multi-user support with unlimited, free user licenses. We provide remote support, additional training and other servic… Experience faster discovery with the suite of live-cell applications from Incucyte®. Learn how the Incucyte® can support your research needs, from setting up an assay to analyzing and exporting your data with a virtual demo This page is also available in your prefered language.Switch to that version.
Incucyte® S3 Applications
- Maintain your cell health with non-perturbing image-based analysis and proprietary reagent formulations.
- Many technologies such as multi-mode readers and high-content imagers do not have the ability to maintain environmental control, meaning cells are not kept at physiologically relevant conditions.
- Traditional end-point assays only provide single measurement of cellular events.
- Cell density should be optimized to prevent contact inhibition, and cells should be allowed to settle post-seeding for even distribution.
- Regular media exchanges every 2-3 days for long assays are essential, unless restricted by the assay.
- Long-term imaging can introduce various artifacts, such as phototoxicity and photobleaching, which can affect cell behavior and data quality.
Live-cell imaging can provide real-time insights into how a patient's cells respond to different treatments, enabling the selection of the most effective therapy. Robotic systems can handle tasks such as media changes, cell feeding, and image acquisition, ensuring consistency and providing researchers the bandwidth to focus on data analysis and interpretation. Capture high-resolution fluorescence and bright-field images of 2D and 3D cell cultures in real time over hours, days, or weeks, directly from the incubator. Incucyte® devices for monitoring and analyzing live cells are designed for efficient recording of cellular changes in the incubator. Researchers can analyze a series of data-points over time, rather than a single time-point that does not provide the full picture of what their cells are doing.
- Fixed cells have been preserved by a fixation step that “locks” the cell in place.
- Fluorescence microscopy enhances the visibility of cellular components by using fluorescent dyes or proteins that emit light when excited by specific wavelengths.
- As such, it becomes easier to target internal cellular structures with dyes and antibodies.
- We are at a very exciting time in the world of science thanks to the advancement of AI software and the utilization of patient-derived samples.
- Live-cell imaging and analysis is the combination of microscopy techniques and powerful analysis software that allows researchers to study spatio-temporal events within cells in real time.
- Easy-to-use software to support all scientists across variety of cell models – turn complex assays into reliable and objective publication-ready data
High-throughput 3D imaging systems will enable large-scale screening of these models, accelerating the discovery of new treatments. This capability could lead to the development of predictive models that forecast cellular responses to treatments, enabling more targeted and effective therapies. AI algorithms can analyze vast amounts of imaging data, identifying patterns and correlations that may not be apparent to human observers. We are at a very exciting time in the world of science thanks to the advancement of AI software and the utilization of patient-derived samples. This technique has the potential to revolutionize our understanding of diseases, drug development, and cellular functions, ultimately contributing to improved healthcare outcomes.
Learn About Live-Cell Imaging & Analysis
Understanding these tradeoffs is essential for designing experiments that yield high-quality data without compromising cell health. Researchers must balance these factors to obtain useful data while maintaining cell viability. There is often a tradeoff between image resolution, acquisition speed, and cell health.
Proper image optimization reduces noise and enhances the signal, making it easier to extract quantitative information from the images. This optimization process is crucial for obtaining clear and accurate images that can be reliably analyzed. A well-defined experimental plan ensures that the study is focused and that the data collected is relevant and meaningful.
Incucyte® devices for Cell Health and Proliferation
By democratizing access to these tools, we can ensure that a wider range of scientists can contribute to and benefit from the advancements in cell biology. 3D cell cultures and organoids more accurately mimic the architecture and function of real tissues, offering a more relevant model for studying disease and drug responses. The continued goatz casino no deposit bonus development of live-cell technologies will allow for the interrogation of 3D samples at scale. Predictive software could potentially determine biological outcomes before they are even visible, revolutionizing the way we understand and treat diseases. The discovery of the cell and the development of microscopes have profoundly impacted human health and quality of life. By minimizing manual intervention, automation enhances consistency and saves time, making it essential for studying dynamic biological phenomena.
Request a complimentary sample of our ready-to-use transfection reagents for life science research involving most mammalian cell lines. Accelerate your next discovery with Incucyte’s® suite of live-cell applications. Reagents have been optimized for each application allowing easy assay preparation and intuitive software that guides the user through each step. These systems again lack proper environmental controls which will impact long-term studies. With increasing technical advancements comes increased complexity and costs; high-resolution microscopes often require extensive training and access is often limited. The timing of adjunct studies such as analysis of metabolites or secreted proteins in supernatants can also be guided.
Purpose-Built Software
These software solutions often include advanced features such as real-time image processing, customizable analysis, and detailed reporting tools. Purpose-built applications can integrate environmental monitoring, automated imaging, and data analysis, providing a seamless experience for researchers. Advanced imaging systems used in live-cell imaging are designed to minimize common issues such as focusing errors and image artifacts. Unlike single-timepoint assays, it enables real-time observation of health, behavior, morphology, and function as cells respond to their environment. The CX3 integrates confocal fluorescence imaging to enable comprehensive monitoring of multicellular 3D cell models throughout discovery and developme… This eBook explores biological live-cell imaging and analysis more deeply
Imaging fixed cells allows high-resolution imaging of sub-cellular structures. See how your peers are using live-cell imaging and analysis Live-cell imaging continues this legacy by providing deeper insights into cellular processes, leading to advancements in medical research and biotechnology.
Maintain your cell health with non-perturbing image-based analysis and proprietary reagent formulations. The Incucyte® itself is not an incubator, but rather sits within a standard tissue culture incubator so cells are maintained in a physiologically relevant environment. Learn about the benefits of AI-driven image analysis and label-free cytotoxicity assays Real-time live-cell analysis is redefining the possibilities and workflows of cell biology. Furthermore, the applications enable analysis of a wide range of phenotypic cell-based assays. With the Incucyte® S3 Live-Cell Analysis System, take your research further with automatic acquisition and analysis of cells in a physiologically relevant environment.
Begin your investigations from the moment cells are placed in culture. Obtain real-time insight into morphological and phenotypic changes for pathway and mechanistic studies by observing time-dependent and cell-dependent treatment effects. Interact with instrument at anytime from anywhere – ideal for routine monitoring of assays and critical for restrictive laboratory conditions Cells remain stationary while optics move below capture changes in biology, without disrupting cell cultures in the stable incubator environment Analyze even the most sensitive living cells around the clock for days, weeks or months.
Discover which live-cell imaging and analysis instrument works best for your lab. Do more with up to five colors specifically designed for live-cell imaging and analysis. Conduct physiologically relevant, robust live-cell imaging and analysis that is streamlined and economical. From 6-well to 384-well microtiter plates to standard tissue culture flasks, the Incucyte® can support your live-cell imaging and analysis needs.
Live Cell Imaging and Analysis Solutions
Maintaining the health and viability of cells over extended periods requires precise regulation of environmental conditions. This level of control is particularly important in complex biological systems where multiple factors can influence outcomes, making it easier to isolate the effects of specific treatments or conditions. The ability to make immediate adjustments based on live data reduces the time and resources spent on trial-and-error, leading to more streamlined and effective experimental designs. This rapid feedback loop accelerates the optimization process, ensuring that assays are fine-tuned for accuracy and efficiency in a shorter time frame. Real-time observation allows researchers to quickly identify and rectify issues in experimental protocols and assay set-up.
With live-cell imaging and analysis, we can monitor changes in real time, which is not possible with typical end-point assays that provide only a snapshot into cellular function. Live-cell imaging and analysis is the combination of microscopy techniques and powerful analysis software that allows researchers to study spatio-temporal events within cells in real time. Live-cell imaging and analysis systems housed within an incubator, such as the Incucyte®, can perform real-time continuous analysis over days to months, while keeping cells in a stable and optimal environment. Live-cell imaging and analysis systems are designed to capture cellular events as they occur in real time. High-content screening and analysis systems are benchtop systems that combine fluorescent microscopy with analysis software to visualize and analyze real-time cellular data.